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IJSTR >> Volume 5 - Issue 12, December 2016 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Cytotoxic Effect Of Verapamil On Human Embryonic Kidney Cell Line

[Full Text]



Jamil L Ahmad, Chukwudi C Okebaram, Abdullahi Samaila



Anti-hypertensives, cancer, growth inhibition, proliferative, verapamil



Introduction: The link between long term use of verapamil and cancer development has been suggested in literature many years back. However, there are numerous controversies surrounding this association with several epidemiological studies in the positive, negative and non-association between verapamil and cancer development. Aim: To investigate in mechanistic terms the link between chronic use of a calcium channel blocker (verapamil) and cancer development using human embryonic kidney (HEK293) cell line. Method: Trypan blue dye exclusion (cell counting) and 3-4, 5-Dimethylthiazol-2-yl-2, 5-diphenyl-tetrazolium bromide (MTT) assays were used to determine the proliferative as well as cytotoxic effects of verapamil. Results: Verapamil had a growth inhibitory rather than proliferative effect on HEK293 cells and the growth inhibition was found to be significant (p<0.05). Conclusion: The long term use of verapamil is associated with cellular growth inhibition and this possibly explained the rationale behind its use as part of combination chemotherapy for some human cancers.



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