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Comparative Analysis Of The Development Of Swarming Communities Of Bacillus Subtilis In Case Of Pta And ComXP Mutant Strains
[Full Text]
AUTHOR(S)
Kassem Hamze, Zaher Zeaiter, Ghayas Lakkis, Ali Shaib, Ahmad Kobaissi
KEYWORDS
Index Terms: Bacillus subtilis, swarming, surfactin, acetyl phosphate, pheromone ComX.
ABSTRACT
Abstract: Swarming Experiments were carried on with Bacillus subtilis strains to identify the activity of certain genes in the swarming ability and surfactin production. We will examine the effect of comXP as well as pta mutations on the capability of swarming. In different experiments we showed that strain OMG 903 that carries mutation in comXP managed to produce surfactin but showed, attenuated defective and random swarming pattern; strain OMG 928 that carries mutation in pta gene, managed to produce surfactin and showed normal swarming pattern, meanwhile double mutation in comXP and pta in strain OMG 929 lead to the absence of surfactin production and didn’t manage Thesetoswarmdatashowed. that a threshold of surfactin production is necessary for a normal swarming pattern.
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