International Journal of Scientific & Technology Research

Home About Us Scope Editorial Board Blog/Latest News Contact Us
10th percentile
Powered by  Scopus
Scopus coverage:
Nov 2018 to May 2020


IJSTR >> Volume 4 - Issue 11, November 2015 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Comparative Analysis Of The Development Of Swarming Communities Of Bacillus Subtilis In Case Of Pta And ComXP Mutant Strains

[Full Text]



Kassem Hamze, Zaher Zeaiter, Ghayas Lakkis, Ali Shaib, Ahmad Kobaissi



Index Terms: Bacillus subtilis, swarming, surfactin, acetyl phosphate, pheromone ComX.



Abstract: Swarming Experiments were carried on with Bacillus subtilis strains to identify the activity of certain genes in the swarming ability and surfactin production. We will examine the effect of comXP as well as pta mutations on the capability of swarming. In different experiments we showed that strain OMG 903 that carries mutation in comXP managed to produce surfactin but showed, attenuated defective and random swarming pattern; strain OMG 928 that carries mutation in pta gene, managed to produce surfactin and showed normal swarming pattern, meanwhile double mutation in comXP and pta in strain OMG 929 lead to the absence of surfactin production and didn’t manage Thesetoswarmdatashowed. that a threshold of surfactin production is necessary for a normal swarming pattern.



[1] K. Hamze, D. Julkowska, S. Autret, K. Hinc, K. Nagorska, A. Sekowska, I.B. Holland and S.J. Séror, “Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC,” Microbiology, Vol. 155, pp. 398–412, 2009.

[2] R.M. Harshey, “Bacterial motility on a surface: many ways to a common goal,” Annu. Rev. Microbiol., Vol. 57, pp. 249–273, 2003.

[3] D. Lopez, H. Vlamakis, R. Losick, and R. Kolter, Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA. Genes & Development, 2009. 23:1631–1638.

[4] N. Verstraeten, K. Braeken, B. Debkumari, M. Fauvart,

[5] J. Fransaer, J. Vermant, and J. Michiels, “Living on a surface: swarming and biofilm formation.” Trends in Microbiol., Vol. 16, pp. 496-508, 2008.
[6] D. Julkowska, M. Obuchowski, I.B. Holland and S.J. Séror, “Comparative Analysis of the Development of Swarming Communities of Bacillus subtilis 168 and a Natural Wild Type,” J. Bacteriol. Vol. 187, pp. 65-76, 2005.

[7] D. Dubnau, “Genetic competence in Bacillus subtilis,” Microbiol Rev., Vol. 55, pp. 395–424, 1991.

[8] D.B. Kearns, and R. Losick, “Swarming motility in undomesticated Bacillus subtilis,” Mol. Microbiol., Vol. 49, pp. 581-590, 2003.

[9] A.H. Klein, A. Shulla, S.A. Reimann, D.H. Keating, and A.J. Wolfe, “The intracellular concentration of acetyl phosphate in Escherichia coli is sufficient for direct phosphorylation of two-component response regulators,” J. Bacteriol., Vol. 189, pp. 5574-5581, 2007.

[10] H. Antelmann, S. Engelmann, R. Schmid, A. Sorokin, Lapidus, and M. Hecker, “Expression of a stress-and starvation-induced dps/pexB-homologous gene is controlled by the alternative sigma factor sigmaB in

[11] Bacillus subtilis,” J. Bacteriol., Vol. 179, pp. 7251–7256, 1997.

[12] K. Hamze, S. Autret, K. Hinc, S. Laalami, D. Julkowska, R. Briandet, M. Renault, C. Absalon, I.B. Holland, H.

[13] Putzer and S.J. Séror, “Single-cell analysis in situ in a Bacillus subtilis swarming community identifies distinct spatially separated subpopulations differentially expressing hag (flagellin), including specialized swarmers,” Microbiology, Vol. 157, pp. 2456–2469, 2011.

[14] S.B. Kim, B.S. Shin, S.K. Choi, C.K. Kim, and S.H. Park, “Involvement of acetyl phosphate in the in vivo activation of the response regulator ComA in Bacillus subtilis,” FEMS Microbiol Lett., Vol. 195, pp. 179–183, 2001.

[15] S. Steller, A. Sokoll, C. Wilde, F. Bernhard, P. Franke and J. Vater, “Initiation of surfactin biosynthesis and the role of the SrfD-thioesterase protein,” Biochemistry, Vol. 43, pp. 11331-11343, 2004.