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IJSTR >> Volume 3- Issue 12, December 2014 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Determination Of Antibacterial Activity And Phytochemistry Of Three Herbal Plants On Clinical Isolates

[Full Text]



Bamidele, F.A., Ogundipe, F.O., Shogeyinbo,U.A



Index Terms: Antibacterial, Phytochemical, Antibiotic susceptibility, Plant extracts.



Abstract: The antibacterial activity of three plants extract Abrus precatorius, Croton penduliflorus (Seeds) and Nauclea latifolia (Root) were evaluated in an in vitro test on Escherichia coli, Klebsiella pneumonia and Enterococcus faecalis. Twelve extracts consisting of hot ethanol, cold ethanol, hot water and cold water were prepared. They were tested using the agar diffusion method. Efficacy was assessed by measuring the diameter of zone of inhibition around the colonies on Muller Hilton agar medium. E.coli showed the highest susceptibility to all plant extracts this was followed by E. faecalis. K. pneumonia showed the least susceptibility to all the extracts. Across board, the cold ethanol extracts of the plants showed the highest susceptibility while the other extracts exhibited variable antibacterial activities. The minimum inhibitory concentration (MIC) of the eight most active extracts ranged between 3.025mg/ml to 50mg/ml while minimum bactericidal concentration (MBC) was between 6.25mg/ml - 25mg/ml. Commonly used antibiotics such as Augmentin and Amoxillin showed low sensitivity as compared to the extracts with varying inhibition zone of 0-4mm Phytochemical screening of these extracts showed that flavonoid was detected in only A. precatorius, Glycoside was found in all extract except in C. penduliflorus extracts. Alkaloid, saponin and Tannin were found in all the plant extracts. This invitro study demonstrated that folk medicine in addition to being cheaper could be effective as modern medicine in combating pathogenic microorganisms.



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