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IJSTR >> Volume 2- Issue 12, December 2013 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Modulation Of Fumonisin B1 Toxic Action-Induced By Zeralenone In Human Intestinal Cells Caco-2

[Full Text]



Kouadio James Halbin, Moukha Serge, Brou Kouakou, Gnakri Dago



Index Terms: Fusarium-toxins, interactive-effect, caspace-3-kinetic-activity, cytotoxicity, oxidative-stress



Abstract: The natural co-occurrence of Fusarium toxins fumonisin B1 (FB1) and Zearalenone (ZEA) in cereal grains is well known. However, a few studies have been reported that address to the toxicity of Fusarium toxins mixtures. Thus, the aim of the present study was to investigate in the interactive effect of binary Fusarium toxins FB1 and ZEA on intestinal cells line Caco-2 using several cellular endpoints such as caspace-3 activity modulation, malonedialdehyde (MDA) production, lactate dehydrogenase (LDH) leakage as necrosis measure and cells viability as evaluated by lysosome and mitochondria integrities. As results, ZEA + FB1 led to an antagonistic effect on lysosome and mitochondria damage but, the necrosis-induced was more potent when compared to ZEA or FB1 alone showing an additive effect. Moreover, MDA production induced by ZEA+FB1 was higher than additive effect but not synergistic effect. At last, the combined effect of toxins on key apoptosis enzyme caspace-3 kinetic activity was an additive effect after 6 h and 24 h but, after 3h, ZEA tended to exert its anti-apoptotic action by reducing the enzyme activation by FB1. Taken together, the results were contrasted and results suggested that combined effects of binary Fusarium toxins ZEA and FB1 in cell line Caco-2 were unpredictable and varied according to several parameters such as the cellular endpoints and the duration of cells incubation with toxins.



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