Effect of Locally Applied Black-seed Oil and Honey Mixture on Wound Healing

Abstract: Back ground: Wound healing is a complex process, which is influenced by multiple factors. Wound management still remains an important focus of researches . The use of natural products as an alternative treatment has been on the rise in the last few decades. blackseed and honey are of the oldest alternative medicine. Objective: The aim of this study is to evaluate the effect of locally applied blackseed oil and honey mixture on experimental wound healing in rabbit. Material and methods: Twenty four young male rabbits from animal house, College of Medicine, University of Mosul were used in this study at 1st of June to 30 of November 2012. The animals divided into two groups after induction of circular wound by 4 mm sharp circular punch in both ears of rabbit under anesthesia . The first group is experimental , the wounds treated daily by local application of black seed oil and honey mixture, the second group is control, no local treatment applied. In the end of 1st , 2nd , 3d , 4th weeks three animals from both group were anesthetized and the wound area and excised. The wound area estimated and the specimens prepared for histological examination for the wounds healing. Results: The mean wound areas in experimental group at the end of 1st, 2nd, 3rd , 4th were 22, 11.7, 7. 5, and 2.3 respectively , while in control group were 22.3, 13.8, 10.5 , and 7 respectively, the difference between two means was significant only at end of 4th week ( p value = 0.003). The histological examination of wound site in experimental group at 1st, 2nd, 3rd, and 4th weeks shows histological scoring 6.12, 8.33, 10. 66, and 11.5 respectively, while in control group 5.66, 7.56, 8.2, and 10.16 respectively. The difference between the two means was significant at the end of 3rd and 4th week. Conclusion: The present study demonstrates that a locally applied blackseed oil and honey mixture enhances healing in experimental wound in rabbit ear.

REFERENCES

[1]. Tan HT, Abdul Rahman R, Gan SH, ET AL.� The antibacterial properties of Malaysian tualang honey against wound and enteric microorganisms in comparison to manuka honey�. BMC Complement Altern Med. 2009; 9: 34.

[2]. Haryanto RN, Tamae U, Kanae M, Suriadi, R, Junko S, Toshio N. � Effectiveness of Indonesian Honey on the Acceleration of Cutaneous Wound Healing�: An Experimental Study in Mice. WOUNDS. 2012;24(4):110�119.

[3]. Kirui PK, Cameron J, Benghuzzi HA, et al.� Effects of sustained delivery of thymoqiunone on bone healing of male rats�. Biomed Sci Instrum. 2004;40:111-6.

[4]. Jull AB, Rodgers A, Walker N.� Honey as a topical treatment for wounds�. Cochrane Database of Systematic Reviews 2008.

[5]. Tan MK, Adli DSH, Tumiran MA, Abdulla MA, Yusoff KM. �The Efficacy of Gelam Honey Dressing towards Excisional Wound Healing�. Evid Based Complement Alternat Med. 2012; 2012: 805932.

[6]. Al-Waili NS, Salom K, Butler G, Al Ghamdi AA. �Honey and microbial infections: a review supporting the use of honey for microbial control�. J Med Food. 2011 ; 14(10): 1079-96.

[7]. Al Mofleh IA, Alhaider AA,1 Mossa JS. Gastroprotective �Effect of an Aqueous Suspension of Black Cumin Nigella sativa on Necrotizing Agents-Induced Gastric Injury in Experimental Animals�. Saudi J Gastroenterol. 2008; 14: 128�134.

[8]. Benhanifia MB, Boukra� L, Hammoudi SM, Sulaiman SA, Manivannan L.� Recent patents on topical application of honey in wound and burn management�. Recent Pat Inflamm Allergy Drug Discov. 2011 Jan;5(1):81-6.

[9]. GuptaS SS, SinghO , Bhagel PS, Moses S , Shukla S, Mathur R K.� Honey dressing versus silver sulfadiazene dressing for wound healing in burn patients�: A retrospective study. J Cutan Aesthet Surg 2011;4:183-7. 2011; 4: 183- 187.

[10]. Oryan A ,S. R. Zaker SR.� Effects of Topical Application of Honey on Cutaneous Wound Healing in Rabbits�. Journal of Veterinary Medicine Series A, 1998; 45: 181- 188.

[11]. Al-Mutheffer EH.� The effect of local application of blackseed (nigella sativa) oil on wound healing in rabbits�. Al-anbar. J, Vet. Sci. 2010; 3: 90- 97.

[12]. Goncalves WLS, Souza FM, Conti JP, et al. �Influence of He- Ne laser therapy on dynamics of healing in mice treated with anti- inflammatory drugs�. Brazilian journal of medical and biological research. 2007; 40: 877- 884.

[13]. Kolbenschlag J, Goertz O, Behr B, Daigeler A, Lehnhardt M, Hirsch T. �Skin antiseptics in plastic surgery� . Handchir Mikrochir Plast Chir. 2012;44(4):254-8.

[14]. Bakhotmah BA, Alzahrani HA.� Self-reported use of complementary and alternative medicine (CAM) products in topical treatment of diabetic foot disorders by diabetic patients in Jeddah�, Western Saudi Arabia. BMC Res Notes. 2010 Oct 6;3:254.

[15]. Alizadeh AM, Sohanaki H, Khaniki M, Mohaghgheghi MA, Ghmami G, Mosavi M.� The Effect of Teucrium Polium Honey on the Wound Healing and Tensile Strength in Rat�. Iran J Basic Med Sci. 2011; 14: 499- 505.

[16]. Mphande AN, Killowe C, Phalira S, Jones HW, Harrison WJ. Effects of honey and sugar dressings on wound healing. J Wound Care. 2007 Jul;16(7):317-9.

[17]. Lee DS, Sinno S, Khachemoune A. Honey and Wound Healing: An Overview. American Journal of Clinical Dermatology. 2011; 12: 181- 190.

[18]. Al-Waili1 NS, Salom K , Al-Ghamdi A. �Honey for Wound Healing, Ulcers, and Burns; Data Supporting Its Use in Clinical Practice� . The Scientific World JOURNAL (2011) 11, 766�787.

[19]. Song JJ, Salcido R.� Use of honey in wound care�: an update. Adv Skin Wound Care. 2011; 24 :40-4.

[20]. Abu-Zinadah OA.� Using Nigella sativa Oil to Treat and Heal Wound of Rabbit Skin�. JKAU: Sci 2009 ; 21: 335-346.

[21]. Abu- Al-basal MA. �In vitro and vivo antibacterial effects of Nigella Sativa seeds extracts against clinical isolates from skin wound infection�. American Journal of Applied Sciences. 2009; 6: 1440- 1447.

[22]. Bakathir HA, Abbas NA. �Detection of the Antibacterial Effect of Nigella Sativa Ground Seeds with Water�. Afr J Tradit Complement Altern Med. 2011; 8: 159�164.

[23]. Wu L, Xia YP, Roth SI, Gruskin E, and Mustoe TA. �Transforming Growth Factor-β1 Fails to Stimulate Wound Healing and Impairs Its Signal Transduction in an Aged Ischemic Ulcer Model� Am J Pathol. 1999 January; 154(1): 301�309.