IJSTR

International Journal of Scientific & Technology Research

Home Contact Us
ARCHIVES
ISSN 2277-8616











 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

IJSTR >> Volume 5 - Issue 10, October 2016 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Study On DPPH* Free Radical - Scavenging Activity Of Antioxidant Compounds In Plants Composing BIN-5 Biological Active Preparation

[Full Text]

 

AUTHOR(S)

Purevjav Urjintseren, Munkhbat Enkhtur, Badamkhand Demberel, Odbayar Tseyen-Oidov

 

KEYWORDS

antioxidant activity, free radicals

 

ABSTRACT

Recently, there has been common trend among people to refuse from food and medications produced via synthetic method, but try to consume natural products as much as possible instead. In this regard, wild berries and medicinal plants are considered to be highly essential for human health as these kinds of plants serve as rich sources of biological active substances-phenol compounds. As a result of conducting research on source and spread of herbs which are commonly used as anti-diabetic medication, we have developed a technological method to extract preparations from medicinal herbs such as Peony (Paeonia lactiflora Pall), Dandelion (Taraxacum officinalis Wigg.), Huckleberry (Vaccinium myrtillus L), Blueberry (Vaccinium uliginosum L), Cranberry (Vaccinium vitisidaea L), and Stinging nettles (Urtica dioica), accordingly studied chemical composition and antioxidant activity, and conducted pharmacological study. With the use of Folin Denis & Folin Ciocalteu reagent method,it was determined that the content of polyphenol compounds was 4.14-5.17% and 27.5 – 101.5mg/ml. The study was also aimed to investigate DPPH* free radical-scavenging activity in connection with term, temperature and concentration to identify the most rational technological procedure. As a result of study, it was identified that free radical-scavenging activity of herbs selected for the study was generally estimated at 564.25-1750.00 mcg/ml, whereas antioxidant activity of solvents with 2-10 mg/ml concentration was 417.20-1750.00 mcg /ml, respectively. This shows that such activity is dependent on concentration. However, in temperature of 30 – 1000С degrees, their activity has slowly been decreased by 1750 mcg/ml – 476.7mcg/ml depending on temperature. Regarding the stinging nettles, the activity was grown directly dependent from temperature. DPHH* free radical-scavenging activity was gradually increased in 1-10 minutes, but was relatively stable and active in 11-16 minutes.

 

REFERENCES

[1] B.Odonbayar, S.Badamtsetseg Study on herbal tea with antioxidant action extracted from medicinal plant of Mongolia

[2] Volodya. Ts. “Medicinal Plants of Mongolia”, 2008. ,345-352.

[3] Dash-Ochir. “Study on food with regulatory actions”, 2011.106-110.

[4] Gogiya.V.Y.,Biochemistry of subtropical plants, 1984., 8,11.

[5] Velioglu, Y.S., Mazza, G., Gao, L. and Oomah, B.D. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J.Agric. Food Chem. 46.

[6] Unursaikhan.S.,Govigerel.B., “Modern methods for determination of antioxidant amount in food”, UB.,2011.

[7] Ligaa.U.,Davaasuren.B., Ninjil.N., “The use of medicinal herbs of Mongolia in western and eastern medicine”, UB., 2005., 99,143.

[8] Determination method of the antioxidant activity in fruit and vegetable by spectrophotometer (MNS 6292: 2011 стандарт)

[9] Markham.K.R., Techiques of flavonoids identification, London, 1982., 113-114.

[10] Ulziikhutag.N., “Guidelines for identification of nutritional plants in pasture of Mongolia”

[11] Singleton.V.L., and Rossi.J.A., Colorimetry of total phenolics with photosphomolybdic-phosphotungstic acid reagents. American Journal of Enology Viticuture1965., 16: 144.

[12] Tserenbaljid.G., “Illustrated gallery of flora of Mongolia”UB.,2002., 62

[13] Gulyaev.V.N.,Technology for food concentrates” , Moscow 1972., 27.

[14] Tsevegsuren.N.,Monkhoobor.D., “Organic chemistry II”, UB., 2013.,401-404.

[15] Andressa Blainski, Gisely Cristiny Lopes and João Carlos Palazzo de Mello, Application and Analysis of the Folin Ciocalteu Method for the Determination of the Total Phenolic Content from Limonium Brasiliense L. 2013.