International Journal of Scientific & Technology Research

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IJSTR >> Volume 5 - Issue 9, September 2016 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616


[Full Text]



Yanti Puspita Sari, Wawan Kustiawan, Sukartiningsih, Afif Ruchaemi



Myrmecodia tuberosa; in vitro; initiation; multiplication; acclimatization.



Propagation of Myrmecodia tuberosa was developed for induction shoot from different explant sources by using various plant growth regulators (PGRs). Cotyledons, hypocotyls, tubers and roots on initiation; shoot tip and axillar bud on multiplication stages have been successfully regenerated on Murashige and Skoog (MS) medium agar. The effects of PGRs BA-6 benzylaminopurine (BAP) on initiation, BAP + GA3-Gibberelic acid (GA3) on multiplication and NAA-α-Naphthalene acetic acid (NAA) on rooting were studied. Various medium both single and combination viz coco peat, fern, charcoal, and moss on acclimatization stage were describe. The results showed that different explant sources of M. tuberosa were potentially produced high number of shootswhich was influenced by the varying concentration of PGRs. In initiation stage, the optimum number of shoots, 15.60 per explant,was obtained from hypocotyls on MS medium agar with BAP 4 mg/L for 12 weeks while axillar bud on MS medium agar BAP 9 mg/L resulted the highest number of shoots (36.60 per explant) for 12 weeks in multiplication stage. In rooting stage, the addition of NAA 0.15 mg/L on MS medium agar was found as optimum PGRs to obtain highest number of root (18.40 root per explant). Plantlets of M. Tuberosa were also successfully acclimatized at green house in coco peat+moss and fern+moss medium in acclimatization with 100% survival. Therefore, it is suggested that the use of specific source e1xplant, PGRs either initial, multiplication, or rooting; and certain combination of medium in acclimation stage are important to be applied in order to increase the population of Myrmecodia tuberosa from North Borneo Island.



[1] Abu-Zahra TR, Al-Shadaideh AN, Abubaker SM, Qrunileh IM. 2013. Influence of auxin concentrations on different ornamental plants rooting. Int J Bot 9:96-99

[2] Achmad H, Armyn SA, Supriatno, Singgih MF. 2014. Anti-cancer activity and anti-proliferation ant nests flavonoid fraction test (Myrmecodya pendans) human tongue cancer cells In Sp-C1 IOSR-JDMS 13:1-5

[3] Anjusha S, Gangaprasad A. 2016. In vitro propagation and anthraquinone quantification in Gynochthodes umbellata (L.) Razafim and B. Bremer (Rubiaceae)—A dye yielding plant Ind Crop Prod 81:83-90 doi:http://dx.doi.org/10.1016/j.indcrop.2015.11.043

[4] Asghari F, Hossieni B, Hassani A, Shirzad H. 2012. Effect of explants source and different hormonal combinations on direct regeneration of basil plants (Ocimum basilicum L.) AJAE 3:12-17

[5] Bhatia S, Dahiya R. 2015. Chapter 4 - concepts and techniques of plant tissue culture science. In: Bera SBSD (ed) Modern applications of plant biotechnology in pharmaceutical sciences. Academic Press, Boston, pp 121-156. doi:http://dx.doi.org/10.1016/B978-0-12-802221-4.00004-2

[6] Deng Z, Jin H, He H. 2015. An efficient micropropagation system for Morinda officinalis how. (Rubiaceae), an endangered medicinal plant J Agr Sci Tech 17:1609-1618

[7] Devadas R, Das J, Naik SK, Medhi RP. 2008. Influence of potting mixture on acclimatization and growth of in vitro developed plantlets of Zygopetalum intermedium J ornamental Hort 11:241-247

[8] Ebrahim N, Shibli R, Makhadmeh I, Shatnawi M, Ein AA. 2007. In vitro propagation and in vivo acclimatization of three coffee cultivars (Coffea arabica L.) from Yemen World Appl Sci J 2:142-150

[9] Fracaro F, Echeverrigaray S. 2001. Micropopagation of Cunila galioides, a popular medicinal plant of South Brazil Plant Cell Tiss Org 64:1-4

[10] George EF, Sherrington PD. 1984. Plant propagation by tissue culture (Handbook and directory of commercial laboratories). Eastern Press, England

[11] Ghatge S, Kudale S, Dixit G. 2011. An improved plant regeneration system for high frequency multiplication of Rubia cordifolia L.: A rare medicinal plant Asia J Biotech 3:397-405

[12] He J, Yu S, Ma C. 2009. Effects of plant growth regulator on endogenous hormone levels during the period of the Red Globe growth J Agr Sci 1:p92

[13] Huxley CR. 1978. The Ant-plants Myrmecodia and Hydnophytum (Rubiaceace), and the relationships between their morphology, ant occupants, physiology and ecology New Phytol 80:231-268

[14] Kadota M, Niimi Y. 2003. Effects of citokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots Plant Cell Tiss Org 72:261-265

[15] Khayyat M, Nazari F, Salehi H. 2007. Effects of different pot mixtures on pothos (Epipremnum aureum Lindl. and Andre ‘Golden Pothos’) growth and development American-Eurasian J Agric & Environ Sci 2:341-348

[16] Kumlay AM, Eryi T. 2011. Growth and development regulators in plants: plant hormones J Inst Sci Tech 1:47-56

[17] Martin K. 2002. Rapid propagation of Holostemma ada-kodien Schult., a rare medicinal plant, through axillary bud multiplication and indirect organogenesis Plant Cell Rep 21:112-117 doi:10.1007/s00299-002-0483-7

[18] Mohamed AN, Ismail MR, Rahman MH. 2010. In vitro respon from cotyledon and hypocotyls explants in tomato by inducing 6-benzylaminopurine Afr J Biotechnol 9:4802-4807

[19] Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497

[20] Nair RR, Kavitha M, Thilaga S, Ganesh D. 2012. Conservation and in vitro multiplicationof higly endangered Indian traditional medicinal plant (Morinda reticulate Gamble) through nodal explants Plant Knowl J 1:46-51

[21] Radha RK, Shereena SR, Divya K, Krishnan PN, Seeni S. 2011. In vitro propagation of Rubia cordifolia Linn. A medicinal plant of the western ghats. Int J Bot 7:90-96

[22] Sevik H, Guney K. 2013. Effects of IAA, IBA, NAA, and GA3 on rooting and morphological features of Melissa officinalis L. stem cuttings Sci World J 2013

[23] Shekhawat MS, Kannan N, Manokari M. 2015. In vitro propagation of traditional medicinal and dye yielding plant Morinda coreia Buch.–Ham S Afr J Bot 100:43-50 doi:http://dx.doi.org/10.1016/j.sajb.2015.05.018

[24] Shekhawat MS, Kannan N, Manokari M, Revathi J. 2012. In vitro propagation of Oldenlandia umbellate L. – A highly medical and dye-yielding plant of coromandel coast. Int J Recent Sci Res 9:758-761
[25] Shi D. 2014. Effects of culture media and plant growth regulators on micropropagation of Willow (Salix matsudana 'Golden Spiral) and Hzelnut (Corylus colurna 'Te Terra Red). University of Nebraska

[26] Shukla S, Shukla SK, Mishra SK. 2009. In vitro plant regeneration from seedling explants of Stereospermum personatum D.C.: a medicinal tree Trees 23:409-413 doi:10.1007/s00468-008-0290-z

[27] Sivanesan I, Hwang SJ, Jeong BR. 2010. Influence of plant growth regulators on axillary shoot multiplication and iron source on growth of Scrophularia takesimensis Nakai-a rare endemic medicinal plant African Journal of Biotechnology 7

[28] Soeksmanto A, Subroto MA, Wijaya H, Simanjuntak P. 2010. Anticancer activity test for extracts of sarang semut plant (Myrmecodia pendens) to HeLa and MCM-Ba cells Pakistan Journal of Biological Sciences 13:148-151

[29] Štefančič M, Štampar F, Osterc G. 2005. Influence of IAA and IBA on root development and quality of Prunus' GiSelA 5'leafy cuttings Hort Science 40:2052-2055

[30] Subroto MA, Saputro H. 2008. Gempur penyakit dengan sarang semut. Penebar Swadaya, Jakarta

[31] Sudrajat H. 2012. Upaya pembibitan biji sarang semut (Myrmecodia pendans) dengan kultur jaringan Agriekonomika 1:47-51

[32] Sudrajat H, Widodo H. 2014. Kultur jaringan tanaman sarang semut (Myrmecodia pendans). (Tissue culture of sarang semut plant). In: Prosiding Seminar Nasional Biodiversitas. Jurusan Biologi. FMIPA-UNS, Solo, Indonesia 2014. pp 67-70

[33] Supriatno. 2014. Antitumor activity of Papua’s Myrmecodia pendans in human oral tongue squamous cell carcinoma cell line through induction of cyclin-dependent kinase inhibitor p27Kip1 and suppression of cyclin E J Cancer Res Ther 2:48-53

[34] Tan Nhut D, Teixeira da Silva JA, Huyen PX, Paek KY. 2004. The importance of explant source on regeneration and micropropagation of Gladiolus by liquid shake culture Scientia Horticulturae 102:407-414 doi:http://dx.doi.org/10.1016/j.scienta.2004.04.004

[35] Victorio CP, Iage CLS, Sato A. 2012. Tissue culture techniques in the proliferation of shoots and roots of Calendula officinales Rev Cienc Agron 43:539-545