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IJSTR >> Volume 4 - Issue 10, October 2015 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

The Study Of The Insecticidal Effect Of Nigella Sativa Essential Oil Against Tuta Absoluta Larvae

[Full Text]



Benchouikh Adil, Allam Tarik, Abderrahim Kribii, Khadija Ounine



Index Terms: Tuta absoluta, Nigella sativa, insecticidal effect, repellent activity.



Abstract: To contribute to the development of an integrated strategy of pests associated with the tomato crop, the insecticidal potential of the essential oil of Nigella sativa was evaluated on larvae of Tuta absoluta, which is considered the most destructive insect in the production of tomato (Solanum lycopersicum) and which causes serious damages in invaded areas. Rearing larvae of Tuta absoluta and the testing of toxicity were conducted in laboratory conditions in petri dishes at a temperature of 26±2°C, with a relative humidity ranging from 60 to 70% and a photoperiod of 16 / 8. The method of Finney based on probits regression of mortalities according to the logarithms of essential oil doses allowed us to determine the LD50. The results of these tests showed that the essential oil of Nigella sativa has remarkable larvicidal properties. After four hours of exposure, they induced 100% of mortality of larvae in the concentration of 0,203μl/cm². The measured LD50 indicated the value of 0,105μl/cm². Field trials are needed to confirm the practical relevance of these results in the development of a natural pesticide against the larvae of Tuta absoluta.



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