|
The Study Of The Insecticidal Effect Of Nigella Sativa Essential Oil Against Tuta Absoluta Larvae
[Full Text]
AUTHOR(S)
Benchouikh Adil, Allam Tarik, Abderrahim Kribii, Khadija Ounine
KEYWORDS
Index Terms: Tuta absoluta, Nigella sativa, insecticidal effect, repellent activity.
ABSTRACT
Abstract: To contribute to the development of an integrated strategy of pests associated with the tomato crop, the insecticidal potential of the essential oil of Nigella sativa was evaluated on larvae of Tuta absoluta, which is considered the most destructive insect in the production of tomato (Solanum lycopersicum) and which causes serious damages in invaded areas. Rearing larvae of Tuta absoluta and the testing of toxicity were conducted in laboratory conditions in petri dishes at a temperature of 26±2°C, with a relative humidity ranging from 60 to 70% and a photoperiod of 16 / 8. The method of Finney based on probits regression of mortalities according to the logarithms of essential oil doses allowed us to determine the LD50. The results of these tests showed that the essential oil of Nigella sativa has remarkable larvicidal properties. After four hours of exposure, they induced 100% of mortality of larvae in the concentration of 0,203μl/cm². The measured LD50 indicated the value of 0,105μl/cm². Field trials are needed to confirm the practical relevance of these results in the development of a natural pesticide against the larvae of Tuta absoluta.
REFERENCES
[1] Chaux C.L. et Foury C.L., Cultures légumières et maraichères. Tome III : légumineuses potagères, légumes fruit .Tec et Doc Lavoisier, Paris, 1994, 563p.
[2] Chibane A., Les cultures maraîchères de l’agroindustrie au Maroc. Bulletin annuel de la division d’horticulture au ministère d’agriculture et de développement rural, 2001.
[3] I.N.P.V., Nouveau déprédateur de la tomate : État des lieux et programme d’action. Note de l’institut national de la protection des végétaux. Ministère de l’agriculture, Algérie, Juillet, 2008.
[4] Desneux N., Wajnberg E., Wyckhuys K. A. G., Burgio G., Arpaia S. C. A., Narváez-Vasquez, González-Cabrera J., Ruescas D. C., Tabone E., Frandon J., Pizzol J., Poncet C., Biological invasion of European tomato crops by Tuta absoluta : ecology, geographic expansion and prospects for biological control. Journal of Pest Science.August, 2010, Vol. 83, Issue 3, pp : 197-215.
[5] Guenaoui Y., Nouveau ravageur de la tomate en Algérie. Première observation de Tuta absoluta, mineuse de la tomate invasive, dans la région de Mostaganem, au printemps 2008. Phytoma défense des végétaux, 2008, Vol.617, pp.18-19.
[6] Picanço M., Leite G.L.D., Guedes R.N.C., Silva E.A., Yield loss in trellised tomato affected by insecticidal sprays and plant spacing. Crop Protection, July 1998, Vol. 17, Issue 5, pp. 447–452.
[7] Lacordaire A.I., Feuvrier E., Tomate, traquer Tuta absoluta. Phytoma La Déf. Vég, 2010, Vol.632, pp.40–44.
[8] Obeng-Ofori D., Reichmuth C.H., Bekele J. & Hassanali A., Biological activity of 1,8-cineole, a major component of essential oil of Ocimum kenyense (Ayobangira) against stored product beetles. Journal of Applied Entomology, 1997, Vol.121, pp.237-243.
[9] Tapondjou L.A., Alder C., Fontem D.A., Bouda H. & Reichmuth C., Bioactivities of cymol and essential oils of Cupressus sempervirens and Eucalyptus saligna against Sitophilus zeamais Motschulsky and Tribolium confusum du Val. Journal of Stored Products Research, 2005, Vol.41, pp.91-102.
[10] Abbott W.S., A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 1925, Vol.18, pp.265-267.
[11] Finney D.J., Statistical Methods in Biological Assay, 2nd edition. London: Griffin, 1971, pp.333.
[12] McDonald L.L., Guy R.H. & Speirs R.D., Preliminary evaluation of new candidate materials as toxicants, repellents and attractants againts stored product insects. Marketing Res. Rep. n° 882. Washington: Agric. Res. Service, US. Dept of Agric., 1970, pp.183.
[13] Casida J.H., Pesticide mode of action, evidence for implications of a finite number of biochemical targets. In: Casida J.E. (ed.). Pesticides and alternatives. Innovative chemical and Biological Approaches to Pest Control. Amsterdam: Elsevier, 1990, pp. 11-22.
[14] Canonica, L., Jommi, G., Scolastico, C., & Bonati, A., The pharmacologically active principle in Nigella sativa. Gazzetta chimica italiana, 1963, Vol.93, pp.1404-1407.
[15] Burits, M., & Bucar, F., Antioxydant activity of Nigella sativa L. essential oil. Phytother Res , 2000, Vol.14, n°5, pp.323-328.
[16] Abdelaziz H. et Catherine R-R., Comparaison des activités insecticides des monoterpènes sur deux espèces d'insectes ravageurs des cultures: Ceratitis capitata et Rhopalosiphum padi. Acta bot. Gallica, 1997, Vol.144, n°4, pp.413-417.
[17] Ojimelukwe P.C. & Alder C., Potential of Zimtaldehyde, 4-allylanisol, linalool, terpinol and others phytochemicals for the control of the confused flour beetle (Tribolium confusum J.D.C.) (G.L. Tenebrionidea). Journal of Pest Science, 1999, Vol.72, pp.81-86.
[18] Agnès F. N., Tapondjou1 A.L., Tendonkeng F., Tchouanguep F. M., 2009, Evaluation des propriétés insecticides des feuilles de Callistemon viminalis (Myrtaceae) contre les adultes d’Acanthoscelides obtectus (Say) (Coleoptera; Bruchidae). TROPICULTURA, 2009, Vol.27, n°3, pp.137-143.
|
