Preliminary Identification Of Banana Peel Indigenous Yeasts With Proteolytic Activity

Banana peel having high content of nutrient such as carbohydrat and protein, can be a source of indigenous yeast with proteolytic activity. This research aims to identify indigenous yeasts with proteolytic activity and antimicrobial activity from banana peel. Banana peel was inoculated into Potato Dextrose Agar (PDA) media modified with 3% yeast extract and 10 ppm Amoxicillin and were incubated for 48 hours in room temperature. Each colony formed were then differentiated macrospically and purified in Yeast Mould Agar (YMA). Purified colonies were further identified under microscope and only the colonies having yeasts morphology were identified using Vitek 2 bioMérieux and tested for its proteolytic activity with paving block method using Nutrient Agar plus 3% Skim Milk, with formation of clear zone were measured as proteolytic activity. Antimicrobial activity tested using well diffusion methods using Nutrient Agar against Salmonella spp. and Escherichia coli. The results showed 2 yeasts isolates found and proteolytic activity was only found in 1 isolate with ±15 mm diameter of clear zone amd antimicrobial activity has showed negative result. The isolate reveales as Candida spp.

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