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International Journal of Scientific & Technology Research

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IJSTR >> Volume 8 - Issue 9, September 2019 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Hydrocarbons Bioremediation Soil Using Eschericia Coli (E. Coli) And Klebsiella SP Bacteria With The Addition Of Inorganic Fertilizers As Nutrients

[Full Text]

 

AUTHOR(S)

Isna Nurul Dukha, Rudi Kartika, Soerja Koesnarpadi

 

KEYWORDS

Bioremediation, TPH, E.coli, Klebsiella sp, Kinetics Bioremediation, Urea Fertilizers, NPK.

 

ABSTRACT

This research studies the effectiveness of TPH bioremediation in oil-exposed soils using E. coli and Klebsiella sp with the addition of urea fertilizer, NPK-Mg 12-12-17 + 2 (N; P; K; Mg = 12%; 12%; 12 %; 2%) and NPK 15-15-15 (N; P; K = 15%; 15%; 15%). The bioremediation process lasts for 3-27 days and during the bioremediation process the TPH level is checked every 72 hours using the InfraCal TPH Analyzer. The results showed that there was an influence on the use of inorganic fertilizers on the length of the bioremediation process, this is because inorganic fertilizers such as urea fertilizer, NPK 12-12-17 + 2 and NPK 15-15-15 contain nutrients needed by bacteria during the bioremediation process so that the bacteria grows optimally. Inorganic fertilizers also affect soil conditions which can cause the bioremediation process to take place faster. The data of this study indicated that the optimal bioremediation conditions were achieved at the highest TPH concentration compared to other TPH concentration variations. This was because pollutants are also a source of carbon for bacterial growth, if the composition of carbon, nitrogen, phosphorus and potassium is available in the appropriate amount for bacterial growth, then bacteria can optimally conduct TPH bioremediation. However, for certain conditions the achievement of bioremediation was achieved in a short time, such as in the bioremediation of 2.21% TPH using E. coli bacteria and NPK 15-15-15 as much as 0.50 grams, the process of decreasing the lowest TPH levels on the 6th day, as well as the use of Klebsiella sp and NPK 12-12-17 + 2 as much as 0.5 grams, a decrease in TPH concentration of 2.21% was reached on the 6th day. This shows that under given conditions, bioremediation is only efficient during the first week. The results of this experiment show the importance of maintaining bacterial biomass by maintaining soil pH and humidity conditions to ensure high bioremediation efficiency. Kinetic form of TPH bioremediation rate in soil concentrations of 8.85%at optimal conditions using E. coli bacteria and the addition of NPK 15-15-15 as much as 0.50 grams, giving a bioremediation rate constant of 0.0560 days-1 with a half-life of 12 days, whereas if using the Klebsiella sp bacteria obtained optimal conditions by using NPK fertilizer 12-12-17 + 2 as much as 0.50 grams and the value of the bioremediation rate constant given is 0.0420 days-1 with a half-life of 17 days.

 

REFERENCES

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