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IJSTR >> Volume 5 - Issue 7, July 2016 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Bioremediation Of Heavy Metals By Pseudomonas Putida Isolated From Groundwater In Egypt.

[Full Text]



Fawazy, G. Khedr, Khalid, A. El-Dougdoug, Khalid, A. Shaban, Essam, I. Abu El Nil



Groundwater, heavy metal resistance bacteria, iron, manganese, transmission electron microscope, Pseudomonas putida, Bioremediation.



In this present study total four bacterial isolates were obtained from 34 collected groundwater samples in 10th of Ramadan, Sharkia governorate, Egypt. These isolate were grown on nutrient agar supplemented with 1mg/l of iron, manganese and combination between them (V/V). Further testing of the bacterial isolates were grown on nutrient agar supplemented with different concentrations (2, 4, 5, 6, 7, 8 and 9 mg/l) of iron and manganese. Out of four isolates, one bacterial isolate no.83 has shown the resistance to heavy metals at maximum concentration of 8mg/l. Selected isolate no.S83 was identified as Pseudomonas putida S83 according to Bergey’s manual depending on morphological and biochemical characteristics. Transmission electron microscopy study of P. putida isolate no. S83 showed accumulation of heavy metal salts within and external to bacterial cells. P. putida S83 have higher removal efficiency of Fe+2 94.5% and Mn+2 94% at concentration 2 mg/l and 96 hours.



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