|
Evaluation Of Susceptibility Of Methanol Extract Of Pleurotus Highking, An Edible Mushroom Cultivated In Bangladesh
[Full Text]
AUTHOR(S)
Md. Anwarul Haque, Ashish Kumar Sarker, Alpana Khatun, Mohammad Sayful Islam¬, Md. Anwar Ul Islam, Abu Syed Md. Anisuzzaman
KEYWORDS
Index Terms: Antibacterial, Antifungal, Kanamycin, Mushroom, Pleurotus highking, Resistance, Susceptibility
ABSTRACT
ABSTRACT: The methanol extract of an edible mushroom, Pleurotus highking with its whole parts, cap and stipe individually were subjected for the evaluation of antimicrobial activities against a series of pathogenic microorganisms. Three Gram positive (Bacillus cereus, Streptococcus agalactiae and Agrobacterium), three Gram negative (Pseudomonas aeruginosa, Escherichia coli and Shigella dysenteriae) and three pathogenic fungus (Aspergillus niger, Saccharromyces cerevaceae and Candida albicans) were used as test pathogen. Disc diffusion assay method was employed for our experimental purpose. All the tested Gram positive strains were sensitive to 200μg of extracts/disc while there was no sensitive to Gram negative strain, Escherichia coli and Shigella dysenteriae. A tested Gram negative strain, Pseudomonas aeruginosa also sensitive to the extracts at a concentration of 200μg/disc. Aspergillus niger and Candida albicans were susceptible to methanol extract of Pleurotus highking while Saccharromyces cerevaceae was resistant to it. However, the susceptibility of the extracts from different parts was varied. The extract from cap showed prominent antimicrobial activity as compared to whole mushroom and stipe.
REFERENCES
[1] Alves, M.J., Ferreira, I.C.F.R., Dias, J., Teixeira, V., Martins, A. and Pintado, M. (2012). A Review on Antimicrobial Activity of Mushroom (Basidiomycetes) Extracts and Isolated Compounds. Planta Med, 78: 1707–1718.
[2] Bala, N., Aitken, E.A.B., Fechner, N., Cusack, A. and Steadman, K.J. (2011). Evaluation of antibacterial activity of Australian basidiomycetous macrofungi using a high-throughput 96-well plate assay. Pharm Biol, 49: 1-9.
[3] Borchers, A., Keen, C.L. and Gershwin, M.E. (2004). Mushrooms, tumors, and immunity: an update. Exp Biol Med, 229: 393-406.
[4] Collins, C.H. and Lyne, P.M. (1987). Microbiological Methods. Butter Worths and Co (Publishers) Ltd., London.
[5] Demirhan, A., Yesil, O.F., Yıldız, A. and Gul, K. (2007). Bazı makrofungus turlerinin antimikrobiyal aktiviteleri uzerine bir arastırma F.Ü. Fen ve Mühendislik Bilimleri Dergisi, 19: 425-433.
[6] Ferreira, I.C.F.R., Vaz, J.A., Vasconcelos, M.H. and Martins, A. (2010). Compounds from Wild Mushrooms with Antitumor Potential. Anticancer Agents Med Chem, 10(5): 424-36.
[7] Kalač, P. (2009). Chemical composition and nutritional value of European species of wild growing mushrooms: A review. Food Chem, 113: 9-16.
[8] Lindequist, U., Niedermeyer, T.H.J. and Jülich, W.D. (2005). The pharmacological potential of mushrooms. eCAM, 2: 285-299.
[9] Nikaido, H. and Vaara, M. (1985). Molecular basis of bacterial outer membrane permeability. Microbiological Reviews, 1: 1–32.
[10] Scherrer, R. and Gerhardt, P. (1971). Molecular sieving by the Bacillum megaterium cell wall and protoplast. Journal of Bacteriology, 107: 718–735.
[11] Tehrani, M.H.H., Fakhrehoseini, E., Nejad, M.K., Mehregan, H. and Hakemi-Vala, M. (2012). Search for Proteins in the Liquid Extract of Edible Mushroom, Agaricusbisporus, and Studying their Antibacterial Effects. Iran J Pharm Res, 11(1): 145–150.
|
