Potential Of Seaweed Padina Sp. As A Source Of Antioxidant

Abstract : The aim of the research was to study the antioxidant activity of crude extract of seaweed Padina sp. The process of extraction of seaweed Padina sp. was done by using maceration stages namely the solvent n-hexane, and then using solvent extraction followed by ethyl acetate and methanol. Solvents that are still left in the third crude extract was evaporated by using a rotary vacuum evaporator, and then tested for DPPH free radical activity (1,1-diphenyl-2-picrylhydrazyl) by UV-VIS spectrophotometer at a wave length of 517 nm. Measurement of antioxidant activity of crude extract of seaweed Padina sp. performed at a concentration of 50 ppm, 100 ppm, 150 ppm, 200 ppm and 250 ppm. Data from tested for DPPH free radical were analyzed using linear regression, than followed by determination of IC50. The test results of all three antioxidant activity of crude extract of seaweed Padina sp. indicated that the higher concentration of solvent, the higher the percentage of arrests of radical activity or percentage inhibition. The antioxidant activity of methanol crude extract of seaweed Padina sp. to give IC50 value of 200.88 mg/L, crude extract of ethyl acetate to give IC50 value of 483.09 mg/L and extract crude n-hexane gave IC50 value of 900.00 mg/L. This means that the antioxidant activity of methanol crude extract of seaweed Padina sp. was stronger than that of crude extract of ethyl acetate and n-hexane.

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