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Phylogenetic Analysis Of Salt Resistant F4 Progeny Of Soybean (Glycine Max (L.) Merril) With Using SSR (Simple Sequence Repeats) Marker
[Full Text]
AUTHOR(S)
Evi Julianita Harahap, Rosmayati, Diana Sofia Hanafiah
KEYWORDS
Progeny F4, soybeans, SSR, saline resistant, phylogenetic
ABSTRACT
Phylogenetic is one of the most commonly used methods in systematics to understand the diversity of living things through the reconstruction of kinship relationships (phylogenetic relationship). SSR marker has some merits such as quickness, simplicity, rich polymorphism and stability, thus being widely applied in genetic diversity analysis, molecular map construction and gene mapping. UPGMA method of phylogenetic analysis is normally the sum of the identical sequences. The objective of this research to find out the genetically diversity and identification of F4 progeny soybean genotype of carrier character salt resistant with used SSR (Simple Sequence Repeats) marker. This research was conducted in Bio molecular Laboratory, Socfindo Seed Production Laboratory (SSPL), Kebun Bangun Bandar Village Martebing District Dolok Masihul Regency Serdang Bedagai on December-May 2017. The number of samples were used 44. Analysis of the data were used software Microsoft Excel 2007, GenAlex ver.6.501, and Multivariate Statistical Package (MVSP) ver.3.2. The UPGMA phylogenetic analysis identified to bring female of 11 plants, male of 3 plants, and both were male and female of 7 plants with coefficient of genetically suitable 0,52.
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