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Characterization Of Bovine Adipose-Derived Stem Cells
[Full Text]
AUTHOR(S)
Daniel Cebo
KEYWORDS
adipocytes, adult stem cells, mesenchymal stem cells, regenerative medicine
ABSTRACT
Bovine adipose-derived stem cells were obtained from the subcutaneous abdominal adipose tissue. The cells were cultured by the modified tissue-explants method developed in our laboratory, and then analyzed using optical microscopy and flow cytometry. These cells were able to replicate in our cell culture conditions. cell Flow cytometry showed that bovine adipose-derived stem cells expressed mesenchymal stem cell markers (CD73 and CD90). Meanwhile, haematopoietic markers (CD45 and CD34) are absent from bovine adipose-derived stem cells. We also induced their adipogenic differentiation in vitro.
REFERENCES
[1] Gregoire, F. M., Smas, C. M. and Sul, H. S. 1988, Physiol. Rev., 78, 783.
[2] Gimble, J. M., Katz, A. J. and Bunnell, B. A. 2007, Circ. Res., 100, 1249.
[3] Mizuno, H., Tobita., M. and Uysal, A. C. 2012, Stem Cells, 30, 804.
[4] Ohsaki, H., Sawa, T., Sasazaki, S., Kano, K., Taniguchi, M., Mukai, F. and Mannen, H. 2007, Comparative Biochemistry and Physiology, 148, 629.
[5] Hirai, S., Matsumoto, H., Moriya, N. H., Kawachi, H. and Yano, H. 2005, Domest. Anim. Endocrinology, 32, 1.
[6] Rodriguez-Menocal, L., Salgado, M., Ford, D. and Van Badiavas, E. 2012, Stem Cells Transl. Med., 1, 221.
[7] Lee, C. H., Shah, B., Moioli, E. K. and Mao, J. J. 2012, J. Clin. Invest., 120, 3340.
[8] Witkowska-Zimny, M. and Walenko, K. 2011, Cell Mol. Biol. Lett., 16, 236.
[9] Avram, M. M., Avram, A. S. and James, W. D. 2007, J. Am. Acad. Dermatol., 56, 472.
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