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IJSTR >> Volume 5 - Issue 5, May 2016 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Sample Preparation In Bioanalysis: A Review

[Full Text]



Anjana Vaghela, Ashok Patel, Ajay Patel, Amit Vyas, Nilesh Patel



Bioanalysis, Liquid chromatography mass spectrometry, Solid phase extraction, Liquid-Liquid extraction, Precipitation



Bioanalysis is sub-discipline of analytical chemistry covering the quantitative measurement of drugs and their metabolites in biological systems. Whereas Liquid chromatography mass spectrometry is a technique which is widely used for the quantification of drug from biological fluid. This article reviews the most recent advances in sample preparation, separation analysis and different type of cartridge used in analysis of biological fluid. Further, this paper also discuss about the merits and demerits of solid phase extraction (SPE), liquid-liquid extraction (LLE) and precipitation.



[1] VP Shah, “The history of bioanalytical method validation and regulation”, American Association Pharma.Science, Vol.9, No. 1, pp.43-47, Feb 2007

[2] C.C. Chan, “Analytical Method Validation and Instrument Performance Verification”, A John Wiley and sons, pp. 105, 2004.

[3] S Murugan, N Pravallika, P Sirisha, K Chandrakala. “A Review on Bioanalytical Method Development and Validation By Using Lc-Ms / Ms.” J. Chem. Pharma Science, Vol. 6, No. 1, pp. 41-45, March 2013

[4] E Reid and ID Wilson,” Analysis for drugs and metabolites,including anti-infective agents: Methodological servey in biochemistry and analysis.”, J. Pharma Pharmacol., Vol. 43, no. 2, pp. 1-57, feb.1991

[5] European medicine agency (EMA) –July 2011,1-23

[6] US Food and drug administration (FDA) – Sept. 2013, 1-34

[7] C. Singleton, “Recent advances in bioanalytical sample preparation for LC–MS analysis.”, Future Science group, Vol. 4, no. 9, pp. 1123-1140, 2012

[8] M. Pawula, G. Hawthorne,GT Smith, HM Hill. ‘Best Practice in Biological Sample Collection , Processing , and Storage for LC-MS in Bioanalysis of Drugs.” A John Wiley and sons, pp. 139-164, 2013

[9] SL Prabu and T Suriyaprakash, “ Extraction of drug from the biological matrix: A review.” Appl Biol Eng Pract, pp.479-507, 2012. Available from: http://www.intechopen.com/books/applied biological-engineering-principlesandpractice/extraction-of-the-drug from-the-biological-matrix assessed on 15/12/2015

[10] Sigma-Aldrich S. Guide to Solid Phase Extraction - bulletin 910. Bull 910 [Internet]. 1998;-. Available from: http://www.sigmaaldrich.com/Graphics/Supelco/objects/4600/4538.pdf

[11] https://waters.com/waters/en_US/Beginner's-Guide-to-SPE-%5BSolid-Phase-Extraction%5D/nav.htm?cid=134721476 assessed on 23/02/2016

[12] M. Henry, SPE technology-Principles and practical consequences. Solid-Phase Extr Princ Tech … [Internet]. 2000; Available from: http://scholar.google.com/scholar?hl=en&btnG=Search&q=intitle:SPE+Technology+-+Principles+and+Practical+Consequences#0 assessed on 28/09/2015

[13] M. Biziuk, “ Solid Phase Extraction Technique – Trends , Opportunities and Applications.” Polish J Environ Stud, vol. 15, no. 5, pp. 677–90, 2006

[14] https://www.waters.com/webassets/cms/support/docs/715000109.pdf assessed on 30/03/2016

[15] http://www.waters.com/waters/partDetail.htm?partNumber=186000252 assessed on 30/03/2016

[16] http://www.waters.com/waters/partDetail.htm?partNumber=186000367 assessed on 30/03/2016

[17] http://www.waters.com/waters/partDetail.htm?partNumber=186002494 assessed on 30/03/2016

[18] http://www.waters.com/waters/partDetail.htm?partNumber=186002493 assessed on 30/03/2016

[19] CH Ball and D P, “Solid Phase Extraction Method Development Tips and Tricks Sources of Error Generated and Time Spent During a Typical Chromatographic Analysis.” Scientist. 2009; available from https://www.agilent.com/cs/library/slidepresentation/Public/Food%20Safety%20Analysis%20Using%20SPE_020309.pdf assessed on 29/03/2016