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IJSTR >> Volume 4 - Issue 5, May 2015 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Levan And Levansucrase-A Mini Review

[Full Text]



Bruna Caroline Marques Goncalves, Cristiani Baldo, Maria Antonia Pedrine Colabone Celligoi



Index Terms: Levan, levansucrase, fermentation, sucrose



Abstract: Levansucrase is a fructosyltranferase that synthesizes levan and present great biotechnological interest. It’s being widely used in therapeutic, food, cosmetic and pharmaceutical industries. Levansucrase is produced by many microorganisms such as the Bacillus subtilis Natto using the sucrose fermentation. In this mini-review we described some properties and functions of this important group of enzymes and the recent technologies used in the production and purification of levansucrase and levan.



[1] M. L. Velázquez-hernandes, V. M. Baizabal-Aguirre, A. Bravo-Patiño, M. Cajero-juárez, M. P. Chávez-moctezuma, J. J. Valdez-Alarcón, “Microbial frusctosyltransferases and the role of fructans”, Journal of Applied Microbiology, vol. 106, pp. 1763-1778, 2009.

[2] R. Chambert, G. Gonzy-treboul, “Levansucrase of Bacillus subtilis: Kinetic and thermodynamic aspects of transfructosylation process”, European Journal of Biochemistry, vol. 62, pp. 55-64,1976.

[3] M. Pascal, F. Kunst, J.-A. Lepesant, R. Dedonder, “Characterization of two sucrase activities in Bacillus subtilis Marburg”, Biochimie, vol. 53, pp. 1059-1066, 1971.

[4] G. Sangiliyandi, P. Gunasekaran, “Polymerase and hydrolase activities of Zymomonas mobilis levansucrase separately modulated by in vitro mutagenesis and elevated temperature”, Process Biochemistry, vol. 36, pp. 543-548, 2001

[5] A. Fout, M. Arnaud, A. Klier, G. Rapoport, “Characterization of the precursor form of the exocellular levansucrase from Bacillus subtilis”, Biochemical and Biophysical Research Communications, vol. 119, pp. 795-800, 1984.

[6] Y. B. Ammar, T. Matsubara, K. Ito, M. Iizuka, T. Limpaseni, P. Pongsawasdi, N. Minamiura, “Characterizacion of a thermostable levansucrase from Bacillus sp. TH4-2 capable of producing high molecular weight levan at high temperature”, Journal of Biotechnology vol. 99, pp. 111-119, 2002.

[7] K. Le Gorrec, C. Connes, A. Guibert, J. L. Uribelarrea, D. Combes, “Identification of three inducible and extracellular enzymatic activities working on sucrose in Bacillus subtilis NCIMB 11871 and 11872 supernatant”, Enzyme and Microbial Technology, vol. 31, pp. 44-52, 2002.

[8] O. Euzenat, A. Guibert, D. Combes, “Production of fructo oligosaccharides by levansucrase from Bacillus subtilis C4”, Process Biochemistry, vol. 32, pp. 237-243, 1997.

[9] M. Bekers, D. Upite, E. Kaminska, M. Grube, J. Laukevics, A. Vigants, P. Zikmanis, “ Fructan biosynthesis by intra and extracellular Zymomonas mobilis levansucrase after simultaneous production of ethanol and levan”, Acta Biotechnology, vol. 23, pp. 85-93, 2003.

[10] C. Martinez-fleitz, M. Ortiz-Lombardía, T. Pons, N. Narbouriech, E. J. Taylor, J. G. Arrieta, L. Hernándes, G. J. Davies, “Cristal structure of levansucrase from the Gram-negative bacterium Gluconocetobacter diazotrophicus”, Biochemistry Journal, vol. 390, pp. 19-27, 2005.

[11] C. R. Dedonder, “Levansucrase from Bacillus subtilis”, Enzymes of Complex Saccharide Synthesis, vol. 86, pp. 500-505, 1966.

[12] R. Chambert, G. Treboul, R. Dedonder, “Kinetic studies of levansucrase of Bacillus subtilis”, European Journal of Biochemistry, vol. 41, pp. 235-300, 1974.

[13] R. Chambert, G. Gonzy-Treboul, “Levansucrase of Bacillus subtilis: Kinetic and thermodynamic aspects of transfructosylation process”, European Journal of Biochemistry, vol. 62, pp. 55-64, 1976.

[14] G. Meng, K. Furtterer, “Structural framework of fructosyl transfer i Bacillus subtilis levansucrase”, Nature Structural Biology, vol. 10, pp. 953-941, 2003.

[15] A. Vigantes, P. Zikmanis, M. Bekers, “Sucrose osmolality as a regulator of anabolic and catabolic parameters in Zymomonas culture”, Acta Biotechnologica, vol. 16, pp. 321-327, 1996.

[16] Vigants, R. Kruce, M. Bekers, P. Zikmanis, “Response of Zymomonas mobilis levansucrase activity to sodium chloride”, Biotechnology Letters, vol. 20, pp. 1017-1019, 1998.

[17] E. Lyness, H. W. Doelle, “Effect of temperature on sucrose to ethanol conversion by Zymomonas mobilis strains”, Biotechnology Letters, vol. 2, pp. 549-554, 1980.

[18] E. Lyness, H. W. Doelle, “Ethanol production from cane juice by Zymomonas mobilis”, Biotechnology Letters, vol. 35, pp. 257-260,1981.

[19] E. Lyness, H. W. Doelle, “Levansucrase from Zymomonas mobilis”, Biotechnology Letters, vol. 5, pp. 345-350, 1983.

[20] G. L. Cote, “Production of a constitutive, extracellular levansucrase from Erwinia herbicola NRRL B-1678”, Biotechnology Letters, vol. 10, p. 879-882, 1988.

[21] A. F. Abdel- Fattah, D. A. R. Mahmoud, M. A. T. Esawy, “ Production of levansucrase from Bacillus subtilis NCR 33A and enzyme synthesis of levan and fructo-oligosaccharids”, Current Microbiology, vol. 55, pp. 402-407, 2005.

[22] S. A. Ahmed, “Optimization of production and extraction parameters of bacillus megaterium levansucrase using solid-state fermentation”, Journal of Applied Sciences Research, vol. 4, pp. 1199-1204, 2008.

[23] H. A. El-Refai, A. F. Abdel-Fattah, F. A. Mostafa, “Immobilization and properties of Bacillus circulans levansucrase”, Acta Pharmaceutica Sciencia, vol. 51, pp. 149-156, 2009.

[24] M. A. Esawy, E. F. Ahmed, W. A. Helmy, N. M. Mansour, W. M El-Senousy, M. M EL-SAFTY, “Production of levansucrase from novel honey Bacillus subtilis isolates capable of producing antiviral levans”, Carbohydrate Polymers, vol. 86, pp. 823-830, 2011.

[25] B.C.M. Gonçalves, J. Mantovan, M.L.L. Ribeiro, D. Borsato, M.A.P.C Celligoi, “Optimization production of thermo active levansucrase from Bacillus subtilis Natto CCT 7712”, Journal of Applied Biology & Biotechnology vol. 1, pp. 1-8, 2013.

[26] T. Tanaka, S. Oi, I. Masaru, T. Yamamoto, “Levansucrase of Bacillus subtilis”, Agricultural Biotechnology Chemistry, vol. 42, pp. 323-326, 1978.

[27] V. K. Ananthalakshmy, P. Guanasekaran, “Isolation and characterization of mutants from levan-producing Zymomonas mobilis”, Journal of Bioscience and Bioengineering, vol. 87, pp. 214-217, 1999 (a).

[28] K. Ananthalakshmy, P. Guanasekaran, “Overproduction of levan in Zymomonas mobilis by using cloned sacB gene”, Enzyme and Microbial Technology, vol. 25, pp. 109-115, 1999 (b).

[29] M. L. Velázquez-Hernandes, V. M. Baizabal-Aguirre, F. Cruz-Vázquez, M. J. Trejo-Contreras, L. E. Fuentes-Ramíres, A. Bravo-Patiño, M. Cajero-Juárez, M. P. Chávez-Moctezuma, J. J. Valdez-Alarcón, “Gluconobacter diazotrophicus levansucrase is involved in tolerance to NaCl, sucrose and desiccation, and in biofilm formation” Archieves of Microbiology, vol. 193, pp. 137-149, 2011.

[30] M. Bekers, D. Upite, E. Kaminska, J. Laukevics, M. Grube, A. Vigants, R. Linde, “Stability of levan produced by Zymomonas mobilis”, Process Biochemistry, vol. 40, pp. 1535-1539, 2005.

[31] Dahech, K. S. Belghith, K Hamden, Feki, A. Antidiabetic activity of levan polysaccharide in alloxan-induced diabetic rats. International Journal of Biological Macromolecules, vol. 40, pp. 742-746, 2011.

[32] J. Leibovici, A. Borit, U. Sandbank, M. Wolman, “The role of macrophages and polymorphs in the levan-induced inhibition of lewis lung carcinoma in C57BL mice”, British Journal of Cancer, vol. 40, pp. 597-606, 1979.

[33] S. H. Yoo, E. J. Yoon, J. Cha, H. G. Lee, “Antitumor activity of levan polysaccharides from selected microorganisms” International Journal of Biological Macromolecules, vol. 34, pp. 37-41 2004.

[34] G.M.T. Calazans, R. C. Lima, F. D. França, C. A. Lopes, “Molecular weight and antitumour activity of Zymomonas mobilis levans”, International Journal of Biological Macromolecules, vol. 27, pp. 245-247, 2000.

[35] L.F. Santos, F.C.B.C Melo, W.J.M. Paiva, D. Borsato, M.L.C Silva, M.A.P.C. Celligoi, “Characterization and optimization of levan production by Bacillus subtilis Natto”, Romanian Biotecnological Letters vol. 18, pp. 8413-8422, 2013.