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IJSTR >> Volume 1 - Issue 4, May 2012 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Molecular Marker Screening For Ty-2 Gene In Two Tomato Genotypes (Solanum Lycopersicum L.)

[Full Text]



Mohamed, S. M., Palchamy, K.



Tomato, TYLCV, molecular screening, phenotypic screening, Ty-2 gene, FLA 456, CLN 2498E



Tomato yellow leaf curl virus (TYLCV) is a major tomato virus in tropical and subtropical regions. The resistance to TYLCV disease in the tomato accession FLA456 and the breeding line CLN2498E was analysed by screening the parents, F1 and BC1F1 population using a sequence tagged site (STS) marker, linked to Ty-2 gene, T0302 at the Biotechnology and Molecular Breeding Unit of the Asian Vegetable Research and Development Centre (AVRDC), the World Vegetable Centre at Taiwan. Results showed that CLN2498E carried Ty-2 gene and FLA456 did not carry it. The BC1F1 plants segregated for Ty-2 gene in a ratio of 46:66 which is closed to 1:1 ( χ=3.33, P< 0.05), which indicated that Ty-2 gene was simply inherited. The phenotypic screening showed that the breeding line CLN2498E was susceptible to TYLCV-Thailand strain although it carried the Ty-2 locus. The accession FLA456 was tolerant to TYLCV-Thailand strain. Although the F1 plants carried the Ty-2 locus, with heterozygous alleles, they showed tolerance to TYLCV-Thailand strain disease. All the plants in the BC1F1 bearing the two bands 900 bp and 800 bp were tolerant to the disease, while others with only 900 bp were susceptible. The results concluded that CLN 2498E carries Ty-2 gene and does not resistant to TYLCV-Thailand strain whereas FLA 456 does not carry Ty-2 gene and resistant to TYLCV-Thailand strain.



[1] P.K. Anderson and F.J. Morales, ‘’Whitefly and whitefly-borne viruses in the tropics: Building a knowledge base for global action,’’ CIAT publication no.341, 351 p,2005.
[2] Y.Bai, C.C. Huang, R. Hults, F. Meijer-Dekens, G. Bonnema and P. Lindhout,’’ QTLs for tomato powdery mildew resistance (Oidium lycopersici) in Lycopersicon parviflorum Gl.1601 co-localized with two qualitative powdery mildew resistance genes,’’ Mol Plant Microbe Interact 16: 196-176, 2003.
[3] A. Barone, ‘\\\'Molecular markers-assisted selection for resistance to pathogens in tomato. Marker Assisted Selection: A fast tract to increase genetic gain in plant and animal breeding,’’ Session1: MAS in plants, 2008.
[4] M.R. Foolad and S. Sharma, ‘’ Solanum genomic network,’’ htt://www.sgn.cornell.edu, 2005.
[5] R.C. Grube, E.R. Radwanski and M. Jahn, ‘’Comparative genetics of disease resistancwithin the Solanaceae.’’ Genetics 155: 873-887, 2000.
[6] P. Hanson, S.K. Green and G. Kuo, ‘’Ty2, a gene on chromosome 11conditioning geminivirus resistance in tomato,’’ Tomato Genetic Cooperative Report 56:17-18, 2006.
[7] Y. Ji, P. Hanson, E. Graham and D.P. Maxwell, ‘’Sources of resistance, inheritance, and location of genetic loci conferring resistance to members of the tomato infection begomoviruses,’’ In: Czosnek H (ed) Tomato yellow leaf curl virus disease: Management, molecular biology, Breeding for resistance. Kluwer, Dordrecht, 2007.
[8] Y. Ji and J.W. Scott, ‘’Ty-3, a begomovirus resistance locus linked to Ty-1 on chromosome 6’’ Report of Tomato Genetic Coop. 56, 2006b.
[9] S.J. Katie, P. Hanson, and M. Luis, ‘’Co-dominant SCAR marker for detection of the begomovirusresistanceTy2 locus derived from Solanum habrochaites in tomato germplasm,’’ (Graham and Hanson, pers. com.)
[10] S.D. Tanksley, M.W. Ganal, J.P. Prince, M.C. Vicente, M.W. Bonierbale, P. Broun, T.M. Fulton, J.J. Giovannoni, S. Grandillo, G.B. Martin, R. Messeguer, J.C. Miller, L.A.H. Miller, O. Paterson, M.S. Pineda, R.A. Roeder, W.Wu. Wing and N.D. Young,’’High density molecular linkage maps of the tomato and potato genomes,’’ Genetics 132:1141-1160, 1992.
[11] D. Zamir, I. Ekstein-Michelson, Y. Zakay, N. Navot, M. Zeidan, M. Sarfatti, Y. Eshed, E. Harel, T. Pleban, H. Van Oss, N. Keder, H.D. Rabinowitch and H. Czosnek, ‘’Mapping and introgression of tomato yellow leaf curl virus tolerance gene, Ty-1,’’ Theor. Appl. Genet. 88:141-146, 1994.