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IJSTR >> Volume 5 - Issue 6, June 2016 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Toxic And Anti-Cancerogenic Effect Of Brown Seaweed Cystoseira Tamariscifolia

[Full Text]



Zineb Souhaili



Acute toxicity, anticancerogenic effect, brown marine algae, Cystoseira tamariscifola, extraction, median lethal dose LD50,



Cystoseira tamariscifolia is a brown alga which represents an important natural biomass on the Moroccan Atlantic coast. Study of the acute toxicity of this species was carried out on Swiss albinos mice. The LD50 was determined through the Spearman-Karber method by injecting the aqueous algal extract to 36 mice organized in lots of 6 animals each for 6 concentrations and the experiments were conducted in triplicates. A dose of 0.5ml of each concentration of the aqueous extract were injected to the mice intraperitonally while the 0.5 ml of saline at 0.9% was injected to the control lot. The anticancerogenic effect study was carried out on murin myelom cells P3/X63-Ag8.653, using different concentrations of the aqueous extract of the alga. The results obtained show a toxic effect of the alga on the mices with LD50 of 738.61±34 µg of alga/ g of body weight. A significant anticancerogenic effect of the extract was also detected on myelom cells; it is propotionnel to the used concentrations.



[1] G. Culioli, C. Vincent-Chabrol, L. Piovetti and R. Valls, ‘’ Etat de la question sur les métabolites secondaires de l’algue brune Bifurcaria bifurcata’’, Cryptogamie Algologie, vol. 19, pp. 257-58, 1998.

[2] J.F. Biard, J.F. Verbst, Y. Letourneux, and R. Floch, ‘’Diterpene ketols with antimicrobial activity from Bifurcaria bifurcata’’, Planta Med. Vol. 40, no. 3, pp.: 288-294, 1980

[3] Z. Kamenarska, F.N. Yalcin, T. Ersoz and I. Calis, ‘’Chemical composition of cystoseira crinita bory from the Eastern Mediterranean’’, Z. Naturforsch,; vol. 57c, pp. 584-590, 2002

[4] G. Hellio, B. Bremer, A.M. Pong, G. Cottenceau, Y. Le Gal and N. Borgougnon, ‘’Inhibition of the development of microorganisms (bacteria and fungi) by extracts of marine algae from Brittany (France)’’, Appl. Microb. Biotech. Vol. 54, no. 4 pp. 543-49, 2002

[5] K. Febles, H. Aria and M. Hardisson, ‘’Antimicrobial activity of extracts from some canary species of phaeophyta and cyanophyta’’, Phytotherapy-Research, vol. 9, no. 5, pp. 385-87, 1995

[6] S. Di Guardia, R. Valls, V. Mesguiche, J.M. Brunel and G. Culioli, “Enantioselective synthesis of (-) –bifurcadiol : a natural antitumor marine product.”, Tetrahedron Letters, vol. 40, pp. 8359-60, 1999

[7] P. Dangeard, ‘’Les algues marines de la côte occidentale du Maroc ‘’, Le botaniste, vol. 34, pp. 89-198, 1949

[8] P. Gayral, ’’Algues de la côte atlantique marocaine’’, La nature au Maroc II , 523p, Rabat, 1958

[9] G. Culioli, S. Di Guardia, S. Valls and L. Piovetti, “Geranylgeraniol-derived diterpenes from the brown alga Bifurcaria bifurcata: Comparison with two other Cystoseiraceae species”, Biochemical Systematics & Ecology, vol. 28, no. 2, pp. 185-87.

[10] G. Culioli, M. Daoudi, A. Ortalo-Magne, R. Valls and L. Piovetti, “ S-(12)-Hydroxygeranylgeraniol-derived diterpenes from the brown alga. Bifurcaria bifurcate”, Phytochemistry, vol. 57, no. 4, pp. 529-35, 2001

[11] H. Hello, G. Thomas-Guyon, L. Culioli, N. Piovetti and N. Bourgougnon, “Marine antifoulants from Bifurcaria bifurcata (Phaeophyceae, Cystoseiraceae) and other brown macroalgae”, Biofouling, vol. 17, no. 3, pp. 189-201, 2001

[12] M. Daoudi, S. Bakkas, G. Culioli, A. Ortalo-Magne, L. Piovetti and M.D. Guiry, “Acyclic diterpenes and sterols from the genera bifurcaria and bifurcariopsis (cystoseiraceae, phaeophyceae)”, Biochemical Systematics and Ecology, vol. 29, pp. 973-978, 2001

[13] A. Culioli, A. Ortalo-Magne, M. Richou, R. Valls and L. Piovetti, “Seasonal variations in the chemical composition of Bifurcaria bifurcata (Cystoseiraceae)”, Biochemical Systematic & Ecology, vol. 30, no. 1, pp. 61-64, 2002

[14] A. Horta, S. Pinteus, C. Alves, N. Fino, J. Silva and S. Fernandez, “Antioxydant and antimicrobial potential of the Bifurcaria bifurcata, epiphytic bacteria”, Mar. Drugs, vol. 12, no. 3, pp. 1676-89, 2014

[15] A. Praud, R. Valls, L. Piovetti, B. Banaigs and G. Malyngamide, ‘’Proposition de structure pour un nouvel amide chloré d’une algue bleue-verte Epiphyte de Cystoseira crinita’’, Tetrahedron Letters, vol. 34, pp. 5437-40, 1993

[16] A Praud, R. Valls, L. Piovetti, B. Banaigs and J. Benaim, “Meroditerpenes from the brown alga Cystoseira crinita of the French Mediterranean coast”. Phytochemistry, vol. 40, no. 2, pp. 495-500, 1995

[17] K.M. Fisch, V. Bohm, A.D. Wright and G.M. Konig, “Antioxydative meroterpenoids from the brown alga Cystoseira crinite”, J. Nat. Prod. Vol. 66 no. 7, pp. 968-75, 2003

[18] A. Bennamara, A. Abourrich, M. Berrada, M. Charrouf, N. Chaib, M. Boudouma and Fx Garneau, “Metoxybifurcarenone : an antifungal and antibacterial meroditerpenoid from the brown alga Cystoseira Tamariscifolia”, Phytochemistry, vol. 52, no. 1, pp. 37-40, 1999

[19] M. El hattab, G. Genta-jouve, N. Bouzidi, A. Orthalo-magné, C. Hellio and J.P. Marechal, “Cystophloroketals A-E, unusual phloroglucinol-Meroterpenoid hybrids from the brown alga Cystoseira tamariscifolia”, J. Nat. Prod. Vol. 78 no. 7, pp. 1663-1670, 2015

[20] L. Custodio, L. Sivestre, M. Rocha, C. vizetto-duarte, H. Pereira and L. Barreira, “Methanol extracts from Cystoseira tamariscifolia and Cystoseira nodicaulis are able to inhibit cholinesterases and protect a human dopaminergic cell line from hydrogen peroxide-induced cytotoxicity”, Pharm. Biol. Vol. 5, pp. 1-10, 2016

[21] A.V.M.A. “Guidelines for the Euthanasia of Animals”, 2013 Edition.

[22] J.A. Gené and A. Robles, ’’Determinación de la dosis letal 50% por el método de Spearman Karber’’, Rev. Med. Hosp. Nal. Niños. Costa Rica, vol. 1, pp. 35-40, 1987

[23] H.C. Hodge and J.H. Sterner, ‘’Determination of substance acute toxicity by LD50’’, American Industrial Hygien Association, vol. 10, pp. 93, 1943

[24] N. Kotake, M. Kushiro, H. Zhang, T. SUGAWARA, K. MIYASHITA and A. NAGAO, ‘’Carotenoids affect proliferation of human prostate cancer cells’’, J. Nutr., vol. 131, no. 12, pp. 3303-6, 2001

[25] S.E. Ayyad, M..O. Salma, A..H. .Mokhtar and A..F. ANTER, “Cytotoxic bicyclc diterpene from the brown alga sargassium crispum”, Biol. .Chim. Fram. Vol. 140, pp. 155-159, 2001

[26] N. Ayyad, O.B. Abdel-Halim, T. Shier and T..R. Hoye, “Cytotoxic hydroazulene diterpenes from the brown alga Cystoseira myrica” Naturforsch., vol. 58c, pp. 33–38, 2003

[27] R. Mokrini†, M. Ben Mesaoud‡, M. Daoudi‡, C. Hellio, J.P. Maréchal, M. El Hattab, A. Ortalo-Magné, L. Piovetti† and G. Culioli, “Meroditerpenoids and Derivatives from the Brown Alga Cystoseira baccata and Their Antifouling Properties”, J. Nat.. Prod. Vol. 71, no. 11, pp. 1806–1811, 2008