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IJSTR >> Volume 5 - Issue 3, March 2016 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Prevalence Rate Of Giardia Lamblia/Helicobacter Pylori Co-Infections In Khartoum State, Sudan

[Full Text]



Nusaiba Fadul Mustafa Ahmed, Tayseer Elamin Mohamed Elfaki, Mohieldin Elsayid



Prevalence rate, Giardia lamblia, Helicobacter pylori, Co-infection.



The study aimed to determine the prevalence rate of Giardia lamblia/ Helicobacter pylori co-infections in Khartoum State, Sudan. A cross-sectional study was carried out during the period between May to December 2015. A total of 100 subjects were included in this study, the age ranging between (1-80) years, the mean age was (29Ī 19) years old. Stool samples were taken from all subjects included in the study, in addition to; clinical and parasitological data were obtained and recorded. Out of 100 subjects, (14 (14%)) were positive for G. lamblia by using direct wet mount and (22 (22%)) were positive by using formal ether concentration technique (FECT) (p=0.000). Out of 100 subjects, (30 (30%)) were positive for H. pylori when detected by using H. pylori antigen test. The study showed that the prevalence of G. lamblia was higher in females (11 (11%)) than in males (3 (3%)) (p=0.193). Also, H. pylori prevalence was higher in females (17 (17%)) than in males (13 (13%)) (p=0.390). The prevalence rate of G. lamblia was higher (5(5%)) in the age group (16- 25) and (46-65) years old by using direct wet mount (p=0.053), while the prevalence rate of H. pylori was higher (9 (9%)) in the age group (1-15) years old (p=0.424). The study revealed that the prevalence rate of G. lamblia and H. pylori co-infections were (5%), (9%) by using direct wet mount and formal ether concentration technique respectively. This study indicated that the prevalence rate of G. lamblia and H. pylori in the study area were (14%), (30%) respectively and co-infection was (5%), with no significant value for co-infection (p=0.615).



[1] A. Samie, R. L. Guerrant, L. Barrett, P. O. Bessong, E. O. Igumbor and C. L. Obi, "Prevalence of Intestinal Parasitic and Bacterial Pathogens in Diarrhoeal and Non diarroeal Human Stools from Vhembe District, South Africa", J Health Popul Nutr, vol.27, no.6, pp.739-745, 2009.

[2] J. Ankarklev, E. Hestvik, M. Lebbad, J. Lindh, D. H. Kaddu-Mulindwa, J. O. Andresson, T. Tylleskar, J. K. Tumwine and S. G. Svard, "Common Co infections of Giardia intestinalis and Helicobacter pylori in Non-Symptomatic Ugandan Children", PLOS Neglected Tropical Diseases, vol. 6 no. 8, pp. 1-8, 2012.

[3] J. W. Ridley, Parasitology for Medical and Clinical Laboratory Professionals. USA, pp. 48-50, 2012.

[4] A. I. Ivanov, "Giardia and Giardiasis", Bulgarian Journal of Veterinary Medicine, vol. 13, no.2, pp. 65-80, 2010.

[5] B. Nkrumah and S. B. Nguah, "Giardia lamblia: a major parasitic cause of childhood diarroea in patients attending a district hospital in Ghana", Parasite and Vectors, vol.4, no. 163, pp. 1-7, 2011.

[6] Y. J. Salman, "Efficacy of some laboratory methods in detecting Giardia lamblia and Cryptosporidium parvum in stool samples", Kirkuk University Journal, vol. 9, no. 1, pp. 7-17, 2014.

[7] B. Chakarova, "Comparative evaluation of the diagnostic methods for detection of Giardia intestinalis in human fecal samples", Trakia Journal of Sciences, vol. 8, no. 2, pp. 174-179, 2010.

[8] M. M. Khalifa, R. R. Sharaf and R. K. Aziz, "Helicobacter pylori: a poor manís gut pathogen", Gut pathogens, vol. 2, no. 2, pp. 1-12, 2010.

[9] L. M. Prescott, J. P. Harley and D. A. Klein. Microbiology, 5th edition, New York, pp. 918-919, 953-954, 2002.

[10] G. F. Brooks, K. C. Carroll, J. S. Butel, S. A. Morse and T. A. Mietzner. Medical Microbiology, 26th edition, USA, pp. 261-262, 719-720, 2013.

[11] O. S. Hamid and W. A. Eldaif, "Association of Helicobacter pylori infection with life style chronic diseases and body-index", Journal of Science, vol. 4, no. 4, pp. 255-258, 2014.

[12] N. A. Abdalsadeg, A. A. Adam, H. Abdul-Aziz, W. H. Omer, H. A. Osman and A. K. Bload, "Comparison of different diagnostic methods of Helicobacter pylori infection in Sudanese patients", NMJ, vol. 2. no. 4, pp. 1-9, 2012.

[13] R. N. Pahwa, V. Kumar and K. Kohli, "Clinical manifestations, causes and management strategies of peptic ulcer disease", IJPSDR, vol. 2, no. 2, pp. 99-106, 2010.

[14] E. Hestvik, T. Tylleskar, D. Mulindwa, G. Ndeezi, L. Grahnquist, E. Olafsdottir and J. K. Tumwine, "Helicobacter pylori in apparently healthy children aged 0-12 years in urban Kampala, Uganda: a community based cross-sectional survey", BMC Gastroenterology, vol. 10, no. 62, pp. 1-9, 2010.

[15] A. Mehmood, M. Akram, S. A. Ahmed, K. Usmanghani, A. Hannan, E. Muhiuddin and M. Asif, "Helicobacter pylori: An Introduction", International Journal of Applied Biology and Pharmaceutical Technology, vol. 1, no. 3, pp. 1337-1351, 2010.

[16] Ministry of Human Development and Labour, "Location and area of Khartoum State",http://www.mhd.gov.sd/English/index.php?option=com_content&view=article&id=248<emid=88. 2015.

[17] R. L. Ichhpujani and R. Bhatia. Medical Parasitology, 3rd edition, New Delhi, pp. 271, 2002.

[18] M. Cheesbrough. District laboratory practice in Tropical Countries, Part 1, Egyptian editions, Cambridge, pp. 193-198, 1998.

[19] A. Lalkhen, A. McCluskey, "Clinical tests: sensitivity and specificity", British Journal of Anaesthesia, vol. 8, no. 6, pp. 221-223, 2008.

[20] E. A. Sabet, H. A. El-hadi, D. S. Mohamed, A. Sheneef, M. Fattouh and M. M. Esmat, "Gastritis; Helicobacter pylori or Giardia lamblia infection or both", Egyptian Journal of Medical Microbiology, vol. 18, no. 4, pp. 165-178, 2009.

[21] L. B. Eltayeb, S. L. Brair and A. S. Aljafari, "The impact of intestinal protozoan parasites among Irritable Bowel Syndrome patients in Khartoum state", NMJ, vol. 3, no. 9, pp. 47-57, 2012.

[22] A. A. Gabbad and M. A. Elawad, "Prevalence of Intestinal Parasite Infection in Primary School Children in Elengaz Area, Khartoum, Sudan", Academic Research International, vol. 5, no. 2, pp. 86-90, 2014.

[23] J.Yakoob, W. Jafri, S. Abid, N. Jafri, S. Hamid, H. Alishah, L. Rizvi, M. Islam and H. Shaikh, "Giardiasis in patients with dyspeptic symptoms", World J Gastroentrol, vol. 11, no. 42, pp. 6667-6670, 2005.

[24] H. H. Raza and R. A. Sami, "Epidemiological study on Gastrointestinal parasites among different sexes, occupations, and age groups in Sulaimani District", J. Duhok Univ., vol. 12, no. 1, pp. 317-323, 2009.

[25] M. O. Mohammed, "Correlation of endoscopic findings with various Helicobacter pylori tests among dyspepsia patients", International Journal of Clinical Medicine, vol. 5, pp. 1180-1188, 2014.

[26] M. A. Bin Mohanna, L. M. Al-zubairi and A. K. Sallam, "Prevalence of Helicobacter pylori and parasites in symptomatic children examined for antibodies, antigens, and parasites in Yemen", Saudi Med. J, vol. 35, no. 11, pp. 1408-1411, 2014.

[27] R. Shafie, M. R. Jahani, M. Rezaeian, A. Amini, A. R. Metvayi, H. Mirahmadi, N. E. Daryani and M. R. Keramati, "Giardia lamblia and Helicobacter coinfection", Iranian J. publ. Health, vol. 38, no. 1, pp. 127-130, 2009.