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IJSTR >> Volume 5 - Issue 5, May 2016 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

The Patterns Of Selected Antibiotics Sensitivity And Resistance To Staphylococcus Aureus Isolates

[Full Text]



Stanslaus Kiilu Musyoki, Stephen Mwaura Kariuki, Kemoi E.K, Elias Mbogori



antibiotics, Azithromycin, Drug resistance; Drug sensitivity, Isolates, penicillin, Staphylococcus aureus



Complications of Staphylococcus aureus infection have greatly increased in recent past because of the many invasive procedures, increased cases of immunocompromised individuals, and the uprising trends in increased antimicrobial resistance of S. aureus strains. Despite of these available information and by contrast with developed countries, S. aureus associated disease are ranked low on the public-health agenda in Kenya and other developing countries. Therefore there is due reason to undertake an investigation and report the trends and patterns in a thorough manner, majorly and especially regarding the antimicrobial resistance. The aim of this study was thus to determine the levels of drug resistance of Staphylococcus aureus to various classes of antibiotics. This data is of significance in improving baseline data on antibiotic resistance of S. aureus isolated from human clinical specimens for the prudent use of antibiotics and the coming up with policies on control programs. All culture isolates were confirmed as Staphylococcus aureus genus by various tests, That is, gram staining, catalase and oxidase. Catalase positive, gram positive and oxidase negative isolates were defined as Staphylococcus. Further analyses by mannitol salt agar fermentation of the isolates and positive coagulase tests indicated Staphylococcus aureus. The area of clearance of sensitivity and tolerance was measured in millimeters and categorized as sensitive, resistant or intermediate. The present study reported that, S. aureus was most sensitive to Azithromycin, whereby 46 (61%) samples were sensitive. Penicillin on the hand was least sensitive showing 29% level of sensitivity. Methicillin, Gentamicin had more than 50% level of sensitivity, That is, 41 (55%) and 40 (53%) respectively. Other antibiotic drugs including ampicillin, augmentin and tetracycline demonstrated less than 50% sensitivity, That is, 29 (39%), 32 (43%) and 33 (44%) respectively. Drug resistance for S. aureus was therefore reported to be highest in penicillin (59%) and least in Azithromycin (25%). Based on results of this study we conclude that drug resistance of Staphylococcus aureus may vary with the antibiotics being used.



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