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IJSTR >> Volume 5 - Issue 3, March 2016 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Studies On Optimization Of Protease Production Using Bacterial Isolate Clri Strain 5468 And Its Application In Dehairing And Hydrolysis Of Tannery Fleshings (Solid Waste Management)

[Full Text]



Vimala Devi Seenivasagham, C. Rose



Protease, unhairing, tannery, hydrolysis, solid waste management



The strain which produces protease was originally isolated, characterized in Biotechnology laboratory at CLRI and was maintained. The microorganism was growned on several proteolytic media and the maximum activity was observed. The characterization of enzyme was analysed for different pH, temperature, size of inoculum, inhibitors, age of the culture. Then the enzyme was observed for the unhairing of skin and the disadvantage in chemical treatment was studied. The conformation of unhairing was studied using histology studies. The tannery waste (solid fleshings) as it is cannot be directly disposed off to the environment. It was treated with the microbial proteases. The hydrolysis of waste was done using proteases. The solid waste was converted to protien, fat and the salt matter. Future work is to optimize the cheap media for the production of the enzyme for large scale applications in various industries.



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