International Journal of Scientific & Technology Research

Home About Us Scope Editorial Board Blog/Latest News Contact Us
10th percentile
Powered by  Scopus
Scopus coverage:
Nov 2018 to May 2020


IJSTR >> Volume 4 - Issue 2, February 2015 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Effect Of Nacl Salt Stress On Antioxidant Enzymes Of Isabgol (Plantago Ovata Forsk.) Genotypes

[Full Text]



Suraj Kala



Key words: Plantago ovata, Superoxide dismutase, Catalase, Peroxidase, NaCl.



Abstract: Activity of antioxidant enzymes such as superoxide dismutase, catalase and peroxidase in leaves of isabgol (Plantago ovata Forsk.) genotypes viz. GI-2, HI-96, PB-80 and HI-5 were studied under salt stress at different EC levels viz. control (without salt), 5 and 10 dSm-1 of nutrient supplemented NaCl salt solutions in sand filled polythene bags. Salt stress caused significant increase in the activity of superoxide dismutase, catalase and peroxidase. Maximum increase in activity of superoxide dismutase and catalase enzymes was found in the genotype GI-2 and minimum increase in the genotype PB-80. Peroxidase activity was highest in the genotype HI-96 and lowest in the genotype PB-80 under salt stress indicating genotype GI-2 and HI-96 having more capacity of scavenging reactive oxygen species produced due to salt stress and were relatively salt tolerant while genotype PB-80 was salt sensitive among the genotypes studied.



[1] Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-126.

[2] Ahmad P, John R, Sarwat M, Umar, S. Response of proline, lipid peroxidation and antioxidative enzymes in two varieties of Pisum sativum L. under salt stress. Int. J. Plant Prod. 2008;2:353-365.

[3] Apel K, Hirt H. Reactive oxygen species: Metabolism oxidative stress and signal transduction. Ann. Rev. Plant Biol. 2004;5:373-399.

[4] Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 1971;44:276-287.

[5] Bor M, Ozdemir F, Turkan I. The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritime L. Plant Sci. 2003;164:77-84.

[6] Bybordi A. Influence of NO3: NH4 ratios and silicon on growth, nitrate reductase activity and fatty acid composition of canola under saline conditions. African J. Agric. Res. 2010;5(15):1984-1992.

[7] Chance B, Maehly AC. Assay of catalase and peroxidase. Methods Enzymol. 1955;2:764-775.

[8] Chaoui A, Mazhoudi S, Ghorbal MH, El Ferjani E. Cadmium and zinc induction of lipid peroxidation and effects of antioxidant enzymes activities in bean (Phaseolus vulgaris L.). Plant Sci. 1997; 127: 139-147.

[9] Chookhampaeng S. The effect of salt stress on growth, chlorophyll content, proline content and anti oxidative enzymes of pepper (Capsicum annuum L.) seedling. European J. Scientific Res. 2011; 49(1):103-109.

[10] Del Rio LA, Corpas FG, Sandalio, LM, Palma JM, Gomez M, Barroso BJ. Reactive oxygen species, antioxidant systems and nitric oxide in peroxisomes. J. Exp. Bot. 2002;531:255-272.

[11] Demiral T, Turkan, I. Comparative lipid peroxidation, antioxidant systems and proline content in roots of two rice cultivars differing in salt tolerance. Environ. Exp. Bot. 2005; 53: 247-257.

[12] Foyer CH, Noctor G. Oxidant and antioxidant signaling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ. 2005;28:1056-1071.

[13] Gama PBS, Tanaka K, Eneji AE, Eltayeb AE, Siddig KE. Salt-induced stress effect on biomass, photosynthetic rate and reactive oxygen species-scavenging enzyme accumulation in common bean. J. Plant Nutr. 2008;32:837-854.

[14] Jaleel CA, Sankar B, Sridharan R, Panneerselvam R. Soil salinity alter growth, chlorophyll content, and the secondary metabolite accumulation in Catharanthus roseus. Turk J. Biol. 2008;32:79-83.

[15] Kahrizi S, Sedghi M, Sofalian O. Effect of salt stress on proline and activity of antioxidant enzymes in ten durum wheat cultivars. Ann. Biol. Res. 2012;3:3870-3874.

[16] Kappus H. Lipid oxidation: mechanism, analysis, enzymology and biological relevance. In: Oxidative stress, ed. H. Sies, 1985; pp. 273-310., London: Academic Press.

[17] Kholova J, Sairam RK, Meena RC, Srivastava GC. Response of maize genotypes to salinity stress in relation to osmolytes and metal-ions contents, oxidative stress and antioxidant enzymes activity. Biol. Plant. 2009;53:249-256.

[18] Latef AAA. Changes in antioxidative enzymes in salinity tolerance among different wheat cultivars. Cereal Res. Comm. 2010;38:43-55.

[19] Maia JM, De-Macedo CEC, Voigt EL, Freitas JBS, Silveira JAG. Antioxidative enzymatic protection in leaves of two contrasting cowpea cultivars under salinity. Biol. Plant. 2010;54:159-163.

[20] Marschner H. Mineral nutrition of higher plants. 2nd ed. Acad. Pr., San Diego. Kindly p. 889. 1995

[21] Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 2002;7:405-410.

[22] Surekha K. Effect of drought and salinity on growth and development of isabgol (Platago ovata Forsk.). 1997; M.Sc. Thesis, CCS Haryana Agricultural University, Hisar, India.

[23] Tappel AL. Lipid peroxidation damage to cell components. Federation Proceedings. 1973;32:1870-1874.

[24] Turkan I, Bor M, Ozdemir F, Koca H. Differencial responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci. 2005;168:223-231.

[25] Vandana N. Physiological response of Isabgol (Plantago ovata Forsk.) genotypes to salt stress. 2003;M.Sc. Thesis, CCS Haryana Agricultural University, Hisar, India.

[26] Varshney UK, Surekha K. Differencial response of chloride and sulphate dominated salinity on growth and developmental aspects of Isabgol (Plantago ovata Forsk.). 2001;Abs. No. 1121. National Symp. on Plant Biodiversity and its conservation. Patiala. P. 56.