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IJSTR >> Volume 10 - Issue 6, June 2021 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Production Of Secondary Metabolites From Heterotrophic Bacteria As Anti-Pathogenic Bacteria

[Full Text]

 

AUTHOR(S)

Jarot Setiaji, Feli Feliatra, Iesje Lukystiowati, Mardalisa

 

KEYWORDS

anti microbes, heterotrophic bacteria, pathogens, Secondary metabolism

 

ABSTRACT

Aquaculture provides a large business opportunity along with the large demand for fish. One problem is the presence of pathogenic bacteria that attack cultivated fish. The use of synthetic antibiotics has been increasingly reduced and returned to natural antimicrobials. The use of natural anti-microbes has great potential to be developed. One of the things that can be used is secondary metabolism of heterotrophic bacteria which is widely spread in the waters. This study aims to determine the ability of secondary metabolite production of heterotrophic bacteria to inhibit the activity of pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas sp).The method used is the experimental method. 10 Heterotrophic bacterial isolates obtained from the collection of marine microbiology laboratories were cultured on Nutrient broth media for 7 days and penned during stationary times, then extracted from secondary media from the media and shells using Etyl Acetate. The secondary metabolite extract was tested against 3 pathogenic bacteria that usually attack fish.Based on the results of the antagonism test conducted, 10 heterotrophic bacterial isolates were able to inhibit the growth of all three pathogenic bacteria. The strength of the inhibition in Vibrio alginolyticus varies between 6.8 - 11.76mm, The strength of the inhibition in Aeromonas hydropila bacteria ranges from 6.8 to 9.5 mm, the strength of the inhibitory strength in Pseudomonas aeroginosa bacteria varies between 6.7-11.1 mm. Overall the inhibition of the three strongest pathogenic bacteria was N isolates (Bacillus cereus code access KM489154.1). and the weakest is Q isolate (Bacillus cereus, code access KY750689.1. Conclusion. Heterotrotic bacteria produce secondary metabolites that can inhibit the growth of pathogenic bacteria in fish. Secondary metabolism of heterotrophic bacteria has the potential to be developed as an anti microbial in pathogenic bacteria in fish..

 

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